Journal: Cancer Cell International

Article Title: Silenced lncRNA DDX11-AS1 or up-regulated microRNA-34a-3p inhibits malignant phenotypes of hepatocellular carcinoma cells via suppression of TRAF5

doi: 10.1186/s12935-021-01847-6

Figure Lengend Snippet: Primer sequence

Article Snippet: The lentivirus containing sh-DDX11-AS1 and sh-NC, miR-34a-3p mimic and its NC were purchased from GenePharma Co. Ltd. (Shanghai, China). pcDDX11-AS1 was constructed by RiboBio Co., Ltd. (Guangdong, China).

Techniques:

Journal: Cancer Cell International

Article Title: Silenced lncRNA DDX11-AS1 or up-regulated microRNA-34a-3p inhibits malignant phenotypes of hepatocellular carcinoma cells via suppression of TRAF5

doi: 10.1186/s12935-021-01847-6

Figure Lengend Snippet: Elevated DDX11-AS1, TRAF5 and reduced miR-34a-3p are present in HCC tissues and cells. a DDX11-AS1, miR-34a-3p and TRAF5 expression in HCC tissues and adjacent tissues detected via RT-qPCR; b TRAF5 protein bands in HCC tissues and adjacent tissues; c TRAF5 protein expression in HCC tissues and adjacent tissues detected via western blot analysis; d The correlation between DDX11-AS1 and miR-34a-3p expression in HCC patients analyzed via Pearson test; e The correlation between DDX11-AS1 and TRAF5 expression in HCC patients analyzed via Pearson test; f The correlation between miR-34a-3p and TRAF5 expression in HCC patients analyzed via Pearson test; g DDX11-AS1, miR-34a-3p and TRAF5 expression in normal human hepatocytes LO2 and HCC cell lines SMMC-7721 and SK-hep1 detected via RT-qPCR; h TRAF5 protein bands in normal human hepatocytes LO2 and HCC cell lines SMMC-7721 and SK-hep1; i TRAF5 protein expression in normal human hepatocytes LO2 and HCC cell lines SMMC-7721 and SK-hep1 detected via western blot analysis; The measurement data were expressed as mean ± standard deviation. The t test was used for two-group comparison. One-way ANOVA was used for comparison among multiple groups, and Tukey’s post hoc test was used for pairwise comparison. Pearson test was used for correlation analysis. * vs LO2 cells, P < 0.05

Article Snippet: The lentivirus containing sh-DDX11-AS1 and sh-NC, miR-34a-3p mimic and its NC were purchased from GenePharma Co. Ltd. (Shanghai, China). pcDDX11-AS1 was constructed by RiboBio Co., Ltd. (Guangdong, China).

Techniques: Expressing, Quantitative RT-PCR, Western Blot, Standard Deviation

Journal: Cancer Cell International

Article Title: Silenced lncRNA DDX11-AS1 or up-regulated microRNA-34a-3p inhibits malignant phenotypes of hepatocellular carcinoma cells via suppression of TRAF5

doi: 10.1186/s12935-021-01847-6

Figure Lengend Snippet: Reduced DDX11-AS1 inhibits the proliferation, promotes apoptosis of HCC cells. a The cell growth curve detected by CCK-8 assay; b The cell proliferation rate detected via colony formation assay; c The apoptosis rate detected via Annexin V-FITC/PI double staining; The measurement data were expressed as mean ± standard deviation. The t test was used for two-group comparison. ^ vs the sh-NC group, P < 0.05

Article Snippet: The lentivirus containing sh-DDX11-AS1 and sh-NC, miR-34a-3p mimic and its NC were purchased from GenePharma Co. Ltd. (Shanghai, China). pcDDX11-AS1 was constructed by RiboBio Co., Ltd. (Guangdong, China).

Techniques: CCK-8 Assay, Colony Assay, Double Staining, Standard Deviation

Journal: Cancer Cell International

Article Title: Silenced lncRNA DDX11-AS1 or up-regulated microRNA-34a-3p inhibits malignant phenotypes of hepatocellular carcinoma cells via suppression of TRAF5

doi: 10.1186/s12935-021-01847-6

Figure Lengend Snippet: Reduced DDX11-AS1 inhibits the migration, invasion of HCC cells, promotes and represses the tumor growth in nude mice. a The cell migration detected by scratch test; b The cell invasion detected by Transwell assay; c Representative images of xenografted tumors in nude mice of each group; d Xenografted tumor volume in nude mice of each group; e Xenografted tumor weight in nude mice of each group; The measurement data were expressed as mean ± standard deviation. The t test was used for two-group comparison. ^ vs the sh-NC group, P < 0.05

Article Snippet: The lentivirus containing sh-DDX11-AS1 and sh-NC, miR-34a-3p mimic and its NC were purchased from GenePharma Co. Ltd. (Shanghai, China). pcDDX11-AS1 was constructed by RiboBio Co., Ltd. (Guangdong, China).

Techniques: Migration, Transwell Assay, Standard Deviation

Journal: Cancer Cell International

Article Title: Silenced lncRNA DDX11-AS1 or up-regulated microRNA-34a-3p inhibits malignant phenotypes of hepatocellular carcinoma cells via suppression of TRAF5

doi: 10.1186/s12935-021-01847-6

Figure Lengend Snippet: Reduced DDX11-AS1 increases miR-34a-3p to reduce TRAF5 expression. a DDX11-AS1, miR-34a-3p and TRAF5 expression detected by RT-qPCR; b TRAF5 protein bands; c TRAF5 protein expression detected by western blot analysis; d The binding sites of DDX11-AS1 and miR-34a-3p predicted via bioinformatics website https://cm.jefferson.edu/rna22/Precomputed/ ; e The regulatory relationship between DDX11-AS1 and miR-34a-3p verified via dual luciferase reporter gene assay; f The binding relationship between DDX11-AS1 and miR-34a-3p verified via RNA-pull down assay; g The targeting relationship between miR-34a-3p and TRAF5 predicted via bioinformatics website https://cm.jefferson.edu/rna22/Precomputed/ ; h The targeting relationship between miR-34a-3p and TRAF5 verified via luciferase activity assay; The measurement data were expressed as mean ± standard deviation. One-way ANOVA was used for comparison among multiple groups, and Tukey’s post hoc test was used for pairwise comparison after ANOVA analysis. ^ vs the sh-NC group, P < 0.05; # vs the mimic NC group, P < 0.05

Article Snippet: The lentivirus containing sh-DDX11-AS1 and sh-NC, miR-34a-3p mimic and its NC were purchased from GenePharma Co. Ltd. (Shanghai, China). pcDDX11-AS1 was constructed by RiboBio Co., Ltd. (Guangdong, China).

Techniques: Expressing, Quantitative RT-PCR, Western Blot, Binding Assay, Luciferase, Reporter Gene Assay, Pull Down Assay, Activity Assay, Standard Deviation

Journal: Cancer Cell International

Article Title: Silenced lncRNA DDX11-AS1 or up-regulated microRNA-34a-3p inhibits malignant phenotypes of hepatocellular carcinoma cells via suppression of TRAF5

doi: 10.1186/s12935-021-01847-6

Figure Lengend Snippet: DDX11-AS1 modulates the growth and metastasis of HCC cells through miR-34a-3p/TRAF5 axis. a , b DDX11-AS1, miR-34a-3p and TRAF5 expression after co-transfection detected by RT-qPCR; c TRAF5 protein expression after co-transfection detected by western blot analysis; d The cell growth curve detected by CCK-8 assay; e The cell proliferation rate detected via colony formation assay; f The apoptosis rate detected via Annexin V-FITC/PI double staining; The measurement data were expressed as mean ± standard deviation. t test was used for comparison between two groups, One-way ANOVA for comparison among multiple groups, and Tukey’s post hoc test for pairwise comparison. & vs the pcDDX11-AS1 + mimic NC group, P < 0.05

Article Snippet: The lentivirus containing sh-DDX11-AS1 and sh-NC, miR-34a-3p mimic and its NC were purchased from GenePharma Co. Ltd. (Shanghai, China). pcDDX11-AS1 was constructed by RiboBio Co., Ltd. (Guangdong, China).

Techniques: Expressing, Cotransfection, Quantitative RT-PCR, Western Blot, CCK-8 Assay, Colony Assay, Double Staining, Standard Deviation

Journal: Cancer Cell International

Article Title: Silenced lncRNA DDX11-AS1 or up-regulated microRNA-34a-3p inhibits malignant phenotypes of hepatocellular carcinoma cells via suppression of TRAF5

doi: 10.1186/s12935-021-01847-6

Figure Lengend Snippet: DDX11-AS1 modulates the growth and metastasis of HCC cells through miR-34a-3p/TRAF5 axis. a , b DDX11-AS1, miR-34a-3p and TRAF5 expression after co-transfection detected by RT-qPCR; c TRAF5 protein expression after co-transfection detected by western blot analysis; d The cell growth curve detected by CCK-8 assay; e The cell proliferation rate detected via colony formation assay; f The apoptosis rate detected via Annexin V-FITC/PI double staining; The measurement data were expressed as mean ± standard deviation. The measurement data were expressed as mean ± standard deviation. t test was used for comparison between two groups, One-way ANOVA for comparison among multiple groups, and Tukey’s post hoc test for pairwise comparison. + vs the pcDDX11-AS1 + sh-NC group, P < 0.05

Article Snippet: The lentivirus containing sh-DDX11-AS1 and sh-NC, miR-34a-3p mimic and its NC were purchased from GenePharma Co. Ltd. (Shanghai, China). pcDDX11-AS1 was constructed by RiboBio Co., Ltd. (Guangdong, China).

Techniques: Expressing, Cotransfection, Quantitative RT-PCR, Western Blot, CCK-8 Assay, Colony Assay, Double Staining, Standard Deviation

Journal: Cancer Cell International

Article Title: Silenced lncRNA DDX11-AS1 or up-regulated microRNA-34a-3p inhibits malignant phenotypes of hepatocellular carcinoma cells via suppression of TRAF5

doi: 10.1186/s12935-021-01847-6

Figure Lengend Snippet: DDX11-AS1 modulates the growth and metastasis of HCC cells through miR-34a-3p/TRAF5 axis. a The cell migration detected by scratch test; b The cell invasion detected by Transwell assay; c Representative images of xenografted tumors in nude mice; d Xenografted tumor volume in nude mice of each group; e Xenografted tumor weight in nude mice of each group; The measurement data were expressed as mean ± standard deviation. The measurement data were expressed as mean ± standard deviation. t test was used for comparison between two groups, One-way ANOVA for comparison among multiple groups, and Tukey’s post hoc test for pairwise comparison. & vs the pcDDX11-AS1 + mimic NC group, P < 0.05

Article Snippet: The lentivirus containing sh-DDX11-AS1 and sh-NC, miR-34a-3p mimic and its NC were purchased from GenePharma Co. Ltd. (Shanghai, China). pcDDX11-AS1 was constructed by RiboBio Co., Ltd. (Guangdong, China).

Techniques: Migration, Transwell Assay, Standard Deviation

Journal: Cancer Cell International

Article Title: Silenced lncRNA DDX11-AS1 or up-regulated microRNA-34a-3p inhibits malignant phenotypes of hepatocellular carcinoma cells via suppression of TRAF5

doi: 10.1186/s12935-021-01847-6

Figure Lengend Snippet: DDX11-AS1 modulates the growth and metastasis of HCC cells through miR-34a-3p/TRAF5 axis. a The cell migration detected by scratch test; b The cell invasion detected by Transwell assay; c Representative images of xenografted tumors in nude mice of each group; d Xenografted tumor volume in nude mice of each group; e Xenografted tumor weight in nude mice of each group; The measurement data were expressed as mean ± standard deviation. The measurement data were expressed as mean ± standard deviation. t test was used for comparison between two groups, One-way ANOVA for comparison among multiple groups, and Tukey’s post hoc test for pairwise comparison. + vs the pcDDX11-AS1 + sh-NC group, P < 0.05

Article Snippet: The lentivirus containing sh-DDX11-AS1 and sh-NC, miR-34a-3p mimic and its NC were purchased from GenePharma Co. Ltd. (Shanghai, China). pcDDX11-AS1 was constructed by RiboBio Co., Ltd. (Guangdong, China).

Techniques: Migration, Transwell Assay, Standard Deviation

Journal: International Journal of Medical Sciences

Article Title: Malignant features related PRDX1 associated with osimertinib sensitivity of EGFR-mutant lung adenocarcinoma

doi: 10.7150/ijms.107255

Figure Lengend Snippet: Primer sequence for qRT-PCR

Article Snippet: The siRNA for PRDX1 was commercially obtained from Ribobio (China).

Techniques: Sequencing

Journal: International Journal of Medical Sciences

Article Title: Malignant features related PRDX1 associated with osimertinib sensitivity of EGFR-mutant lung adenocarcinoma

doi: 10.7150/ijms.107255

Figure Lengend Snippet: Expression of PRDX genes in LUAD at protein level. (A-F) IHC images were acquired using HPA, which concerns protein levels of PRDX1, PRDX2, PRDX3, PRDX4, PRDX5, and PRDX6 expressed in LUAD and normal samples.

Article Snippet: The siRNA for PRDX1 was commercially obtained from Ribobio (China).

Techniques: Expressing

Journal: International Journal of Medical Sciences

Article Title: Malignant features related PRDX1 associated with osimertinib sensitivity of EGFR-mutant lung adenocarcinoma

doi: 10.7150/ijms.107255

Figure Lengend Snippet: Drug sensitivity comparison, molecular docking, and expression exploration of PRDX1. (A) CTRP drug sensitivity profiles are illustrated from the top to the bottom according to the correlation between each PRDX member and the IC 50 of each CTRP drug. (B) Expression of PRDX1-3 in EGFR wild-type and mutant LUAD based on the GSE31210 dataset. (C) Visualization of the pocket structure and spatial arrangement of PRDX1 interaction with osimertinib. (D) Energy map of the interaction between PRDX1 and osimertinib. (E) Expression levels of PRDX1 in 25 EGFR LUAD samples, 13 EGFR-mutant samples, and 12 EGFR wild-type LUAD samples compared with that in the adjacent lung tissue, and expression of PRDX1 in the EGFR-mutant samples compared with the EGFR wild-type samples. * P < 0.05 compared with control.

Article Snippet: The siRNA for PRDX1 was commercially obtained from Ribobio (China).

Techniques: Comparison, Expressing, Mutagenesis, Control

Journal: International Journal of Medical Sciences

Article Title: Malignant features related PRDX1 associated with osimertinib sensitivity of EGFR-mutant lung adenocarcinoma

doi: 10.7150/ijms.107255

Figure Lengend Snippet: Co-expressed genes and pathway enrichment of PRDX1. (A) Volcano plot showing genes positively and negatively correlated with PRDX1 expression obtained from cBioportal website. (B) PPI network plot created by cytoscape software showing the interaction network of the top 84 genes correlated with PRDX1. (C) Bubble plot showing GO (including BP, CC, MF) and KEGG pathway enrichment of genes significantly correlated with PRDX1 expression. (D) The GSEA pathway, which is positively correlated with PRDX1, is enriched for DNA replication, cell cycle checkpoints, homology directed repair, TP53 regulates transcription of DNA repair genes. NES, normalised enrichment scoring.

Article Snippet: The siRNA for PRDX1 was commercially obtained from Ribobio (China).

Techniques: Expressing, Software

Journal: International Journal of Medical Sciences

Article Title: Malignant features related PRDX1 associated with osimertinib sensitivity of EGFR-mutant lung adenocarcinoma

doi: 10.7150/ijms.107255

Figure Lengend Snippet: Detection of expression of PRDX1 in LUAD cells and the IC 50 of LUAD cells for osimertinib. (A) Relative expression of PRDX1 in different LUAD cell lines and in human normal lung epithelial cells, U6 was used as the nuclear internal reference. (B) mRNA knockdown efficiency of PRDX1. (C) Dose-dependent cytotoxicity of osimertinib in A549 cell lines. (D) Dose-dependent cytotoxicity of osimertinib in PC-9 cell lines (OD value=450 nm). Data are presented as mean ± SD; * p < 0.05, ** p < 0.01, *** p < 0.001 by Student's t -test.

Article Snippet: The siRNA for PRDX1 was commercially obtained from Ribobio (China).

Techniques: Expressing, Knockdown

Journal: International Journal of Medical Sciences

Article Title: Malignant features related PRDX1 associated with osimertinib sensitivity of EGFR-mutant lung adenocarcinoma

doi: 10.7150/ijms.107255

Figure Lengend Snippet: Impact of PRDX1 knockdown on the sensitivity of EGFR-mutant and wild-type LUAD cells for osimertinib. (A-E) Impact of PRDX1 on the sensitivity of A549 and PC-9 cells for osimertinib was assessed by proliferation activity through EdU uptake assays (Scale bar: 100 μm), and colony formation test (colonies >50 μm counted as positive). Data are presented as mean ± SD; * p < 0.05, ** p < 0.01, *** p < 0.001 by Student's t -test.

Article Snippet: The siRNA for PRDX1 was commercially obtained from Ribobio (China).

Techniques: Knockdown, Mutagenesis, Activity Assay

Journal: International Journal of Medical Sciences

Article Title: Malignant features related PRDX1 associated with osimertinib sensitivity of EGFR-mutant lung adenocarcinoma

doi: 10.7150/ijms.107255

Figure Lengend Snippet: Effect of presence/absence PRDX1 on osimertinib-induced apoptosis of EGFR-mutant and wild-type LUAD cells. (A) Detection of PRDX1 knockdown on osimertinib- induced apoptosis of A549 cells. (B) Detection of PRDX1 knockdown on osimertinib-induced apoptosis of PC-9 cells. Data are presented as mean ± SD; * p < 0.05, ** p < 0.01, *** p < 0.001 by Student's t -test.

Article Snippet: The siRNA for PRDX1 was commercially obtained from Ribobio (China).

Techniques: Mutagenesis, Knockdown

Journal: International Journal of Medical Sciences

Article Title: Malignant features related PRDX1 associated with osimertinib sensitivity of EGFR-mutant lung adenocarcinoma

doi: 10.7150/ijms.107255

Figure Lengend Snippet: Association between the expression of PRDX1 and immune cells infiltration . (A) TME cell types and corresponding cell distributions in the GSE131907 set were obtained from the TISH database. (B ) Distribution of PRDX1 in various cell types of the TME at single-cell resolution. (C) Violin plots demonstrating PRDX1 expression in various cell types of TME. (D) Correlation of PRDX1 expression with the infiltration of M2 macrophages, mast cells, monocytes, plasma cells, and DCs obtained using the TMIER2.0 website tool. (E) Correlation of PRDX1 expression with molecular markers of DCs, including HLA-DQB1, HLA-DPB1, HLA-DRA, HLA-DPA1, CD1C, and NRP1. Correlation analyses were statistically performed using the Spearman algorithm.

Article Snippet: The siRNA for PRDX1 was commercially obtained from Ribobio (China).

Techniques: Expressing, Clinical Proteomics